Please use this identifier to cite or link to this item:
http://arks.princeton.edu/ark:/88435/dsp01jm214s26c
Full metadata record
DC Field | Value | Language |
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dc.contributor.advisor | Ploss, Alexander | - |
dc.contributor.author | Madan, Vrinda | - |
dc.date.accessioned | 2021-08-20T15:14:05Z | - |
dc.date.available | 2023-07-03T12:00:08Z | - |
dc.date.created | 2021-04-16 | - |
dc.date.issued | 2021-08-20 | - |
dc.identifier.uri | http://arks.princeton.edu/ark:/88435/dsp01jm214s26c | - |
dc.description.abstract | Dengue virus (DENV), a potentially life-threatening disease affecting millions each year, remains a major global health threat. The scarcity of animal models mimicking human symptoms of DENV infection has slowed our understanding of disease pathogenesis and development of anti-viral therapies. Here, we investigate the use of previously established humanized HIS-NFA2/hFLT3LG mice to model immune responses following DENV infection. We first generated a diverse library of 20 infectious clones based on patient-derived viral strains—serving as an important tool for both this study and subsequent characterizations. Using two of these low-passage DENV clinical isolates, we demonstrate that HIS-NFA2/hFLT3LG mice can support viral replication. Additionally, these humanized mice display key clinical symptoms of DENV, including viremia and thrombocytopenia. We further characterized DENV-infected HIS-NFA2/hFLT3LG mice on the basis of their cellular and humoral immunity, finding that DENV-specific responses were activated similar to those seen in patients. In summary, we demonstrate that HIS-NFA2/hFLT3LG mice are susceptible to infection with clinical DENV clinical isolates, mount virus-specific adaptive immune response, and mimic key disease manifestations. Although additional refinements to the model are required, we anticipate that our HIS-NFA2/hFLT3LG system will be widely useful to mechanistically study DENV pathogenesis and for assessing the efficacy of anti-DENV therapeutics. | en_US |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | en_US |
dc.title | Characterization and Optimization of Primary Dengue Viral Infection in a Humanized Mouse Model | en_US |
dc.type | Princeton University Senior Theses | |
pu.embargo.terms | 2023-07-01 | - |
pu.date.classyear | 2021 | en_US |
pu.department | Molecular Biology | en_US |
pu.pdf.coverpage | SeniorThesisCoverPage | |
pu.contributor.authorid | 920191706 | |
pu.certificate | Global Health and Health Policy Program | en_US |
pu.mudd.walkin | Yes | en_US |
Appears in Collections: | Molecular Biology, 1954-2024 Global Health and Health Policy Program, 2017-2023 |
Files in This Item:
File | Description | Size | Format | |
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MADAN-VRINDA-THESIS.pdf | 2.83 MB | Adobe PDF | Request a copy |
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