Skip navigation
Please use this identifier to cite or link to this item:
Title: Understanding the Metabolic Differences of Osteochondritis Dissecans From Horse Blood Serum Samples
Authors: Essani, Insiya
Advisors: Pelczer, Istvan
Department: Chemistry
Certificate Program: Global Health and Health Policy Program
Class Year: 2020
Abstract: Osteochondritis dissecans is a common cause of degenerative joint disease in all horse breeds. Given the similarity between human and horse cartilage, horses serve as model organisms to understand translatable metabolic differences. 1H NMR spectroscopy is a powerful tool commonly used to identify metabolites. However, most metabolomic studies limit their focus on small molecules by conducting relaxation filtered experiments. This study focuses on expanding the 1H NMR experiments that are routinely used. Through these additional experiments, a more complete metabolic profile is deduced consisting of both macromolecules and small molecules. This study carefully processes NMR data for each experiment using MestReNova and develops a processing template. Metabolites found in OCD horse blood serum samples are assigned. Multivariate analysis in SIMCA creates OPLS-DA models that reveal separation between the two classes. Coefficient plots used in conjunction with the original spectra reveal the most significant metabolic variations. Significant variations were not limited to small molecules, and future studies should focus on understanding metabolic differences due to lipoprotein subgroups. Observations from these coefficient plots set the foundation to investigate casual relationships between metabolites and OCD.
Type of Material: Princeton University Senior Theses
Language: en
Appears in Collections:Chemistry, 1926-2022
Global Health and Health Policy Program, 2017-2022

Files in This Item:
File Description SizeFormat 
ESSANI-INSIYA-THESIS.pdf11.02 MBAdobe PDF    Request a copy

Items in Dataspace are protected by copyright, with all rights reserved, unless otherwise indicated.