Skip navigation
Please use this identifier to cite or link to this item: http://arks.princeton.edu/ark:/88435/dsp01zc77ss72p
Full metadata record
DC FieldValueLanguage
dc.contributor.advisorAndo, Nozomi-
dc.contributor.authorAnzai, Isao-
dc.date.accessioned2017-07-19T13:36:38Z-
dc.date.available2019-07-01T09:15:51Z-
dc.date.created2017-04-28-
dc.date.issued2017-4-28-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/dsp01zc77ss72p-
dc.description.abstractOur present society’s rampant consumption of coal, oil, and natural gas will leave us not only largely bereft of fossil fuel stocks by the year 2100 but also mired in environmental, societal, and health concerns impacting all segments of civilization. Our desire to mitigate these problems opens up a variety of possibilities in the way of sustainable energy. One especially promising solution, known as electrosynthesis, seeks to merge the energy capture efficiency of modern-day photovoltaics with the metabolic flexibility of electroactive microorganisms that are capable of harnessing photovoltaic electrons by extracellular electron transfer (EET). Currently, however, our largest obstacle hindering our ability to maximize the efficiency of electrosynthesis for widespread use is our incomplete understanding of the genes and genetic networks underlying EET. To address this concern, our lab invented Knockout Sudoku, a novel method relying on transposon mutagenesis for creating highly complete and annotated whole genome knockout collections. With this technique, we created a knockout collection of the electroactive bacterium Shewanella oneidensis and have begun to extract valuable information concerning the genetic basis behind EET. This work and future research, including the potential to create many more knockout collections at a low-cost and rapid scale, will inform our ability to apply biological organisms toward a more sustainable future in energy.en_US
dc.language.isoen_USen_US
dc.titleKnockout Sudoku: An Investigation of the Genetic Basis Behind Extracellular Electron Transfer in Shewanella oneidensis MR-1en_US
dc.typePrinceton University Senior Theses-
pu.embargo.terms2019-07-01-
pu.date.classyear2017en_US
pu.departmentMolecular Biologyen_US
pu.pdf.coverpageSeniorThesisCoverPage-
pu.contributor.authorid960831530-
pu.contributor.advisorid960002642-
pu.certificateGlobal Health and Health Policy Programen_US
pu.mudd.walkinyesen_US
Appears in Collections:Molecular Biology, 1954-2023
Global Health and Health Policy Program, 2017-2023

Files in This Item:
File SizeFormat 
Senior_Thesis_Final.pdf9.13 MBAdobe PDF    Request a copy


Items in Dataspace are protected by copyright, with all rights reserved, unless otherwise indicated.