3D Nano-printed Landscapes and Interfaces for Biomedical Applications

Abstract

The goal of this report was to determine how well different photoresins worked with cells and to characterize the resins through stress and resolution tests. From these results, it would then be possible to determine how best to apply these resins in a biomedical context (e.g., which resin would be best for exploring tissue-integration methods). The resins used were non-cytotoxic resins produced by Nanoscribe, specifically, IP-L, IP-S, and IP-Visio. The first test was an adhesion test, and results showed that cells adhered to all surfaces, though they were slower to spread on the IP-Visio. It was also found that, after 18 hours, the patch made from IP-Visio began to peel off of the substrate it had been printed upon, possibly due to the parameters used not being suitable for printing with IP-Visio. The second test was a small stress/resolution test that was first conducted with the IP-L, and the result of that was a print that had a lot of bubbles. A parameter sweep of laser powers and scan speeds was then conducted to determine what values would prevent these bubbles from appearing again. From the parameter sweep, it was found that the ideal laser power should be between 80-100\% and the ideal scan speed should be between 60,000-80,000 $\mu$m/s.