Please use this identifier to cite or link to this item:
http://arks.princeton.edu/ark:/88435/dsp01qz20sw62h
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yan, Nieng | - |
dc.contributor.author | Kim, Minyoung | - |
dc.date.accessioned | 2021-08-20T17:53:19Z | - |
dc.date.available | 2021-08-20T17:53:19Z | - |
dc.date.created | 2021-04-16 | - |
dc.date.issued | 2021-08-20 | - |
dc.identifier.uri | http://arks.princeton.edu/ark:/88435/dsp01qz20sw62h | - |
dc.description.abstract | The throughput of electron cryo-microscopy (cryo-EM) can be improved by collecting data using beam-image shift instead of the traditional stage-shift method. Despite successful implementation of beam-image shift on various cryo-EM instruments, it remains unknown how beam-image shift affects the quality of data collected on spherical aberration (Cs)-corrected cryo-EM instruments. To investigate this, a cryo-EM dataset for Thermoplasma acidophilum 20S (T20S) proteasome was collected on a 300 kV Cs-corrected transmission electron microscope (TEM) using beam-image shift. Analyzing the dataset in two different image-processing software, RELION and CryoSPARC demonstrated that there are minimal differences between the resolutions of T20S structures generated from datasets collected using with and without beam-image shift. In addition, findings of this study demonstrated that data collected using with and without beam-image shift can be combined and processed together to correct for image abberations. | en_US |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | en_US |
dc.title | BEAM IMAGE-SHIFT ACCELERATED DATA ACQUISITION FOR SINGLE-PARTICLE ELECTRON CRYO-MICROSCOPY WITH SPHERICAL ABERRATION CORRECTION | en_US |
dc.type | Princeton University Senior Theses | |
pu.date.classyear | 2021 | en_US |
pu.department | Molecular Biology | en_US |
pu.pdf.coverpage | SeniorThesisCoverPage | |
pu.contributor.authorid | 920191731 | |
pu.mudd.walkin | No | en_US |
Appears in Collections: | Molecular Biology, 1954-2023 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
KIM-MINYOUNG-THESIS.pdf | 2.21 MB | Adobe PDF | Request a copy |
Items in Dataspace are protected by copyright, with all rights reserved, unless otherwise indicated.