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Please use this identifier to cite or link to this item: http://arks.princeton.edu/ark:/88435/dsp01qz20sw62h
Title: BEAM IMAGE-SHIFT ACCELERATED DATA ACQUISITION FOR SINGLE-PARTICLE ELECTRON CRYO-MICROSCOPY WITH SPHERICAL ABERRATION CORRECTION
Authors: Kim, Minyoung
Advisors: Yan, Nieng
Department: Molecular Biology
Class Year: 2021
Abstract: The throughput of electron cryo-microscopy (cryo-EM) can be improved by collecting data using beam-image shift instead of the traditional stage-shift method. Despite successful implementation of beam-image shift on various cryo-EM instruments, it remains unknown how beam-image shift affects the quality of data collected on spherical aberration (Cs)-corrected cryo-EM instruments. To investigate this, a cryo-EM dataset for Thermoplasma acidophilum 20S (T20S) proteasome was collected on a 300 kV Cs-corrected transmission electron microscope (TEM) using beam-image shift. Analyzing the dataset in two different image-processing software, RELION and CryoSPARC demonstrated that there are minimal differences between the resolutions of T20S structures generated from datasets collected using with and without beam-image shift. In addition, findings of this study demonstrated that data collected using with and without beam-image shift can be combined and processed together to correct for image abberations.
URI: http://arks.princeton.edu/ark:/88435/dsp01qz20sw62h
Type of Material: Princeton University Senior Theses
Language: en
Appears in Collections:Molecular Biology, 1954-2021

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