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Title: Data For “Development of a dual channel detection system for pan-genotypic simultaneous quantification of hepatitis B and delta viruses"
Contributors: Maya, Stephanie
Carver, Sebastian
O’Connell, Aoife
Zen, Anna
Gertje, Hans
Seneca, Kathleen
Nahass, Ronald
Crossland, Nicholas
Ploss, Alexander
Liu, Yongzhen
Keywords: hepatitis delta virus
hepatitis B virus
viral hepatitis
viral detection
humanized mice
Issue Date: 2024
Publisher: Princeton University
Abstract: Hepatitis B virus (HBV) infection remains a major public health problem and, in associated co-infection with hepatitis delta virus (HDV), causes the most severe viral hepatitis and accelerated liver disease progression. As a defective satellite RNA virus, HDV can only propagate in the presence of HBV infection, which makes HBV DNA and HDV RNA the standard biomarkers for monitoring the virological response upon antiviral therapy, in co-infected patients. Although assays have been described to quantify these viral nucleic acids in circulation independently, a method for monitoring both viruses simultaneously is not available, thus hampering characterization of their complex dynamic interactions. Here, we describe the development of a dual fluorescence channel detection system for pan-genotypic, simultaneous quantification of HBV DNA and HDV RNA through a one-step quantitative PCR. The sensitivity for both HBV and HDV is about 10 copies per microliter without significant interference between these two detection targets. This assay provides reliable detection for HBV and HDV basic research in vitro and in human liver chimeric mice. Preclinical validation of this system on serum samples from patient on or off antiviral therapy also illustrates a promising application that is rapid and cost-effective in monitoring HBV and HDV viral loads simultaneously.
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20240429-DATASET_Development of a dual channel detection system for pan-genotypic simultaneous quantification of hepatitis B and delta viruses copy.txtREADME file40.08 kBTextView/Download
Fig 1B.csv352 BCSVView/Download
Fig 1C.csv362 BCSVView/Download
Fig 1D.csv464 BCSVView/Download
Fig 1F.csv506 BCSVView/Download
Fig 1G.csv823 BCSVView/Download
Fig 1H.csv732 BCSVView/Download
Fig 1I.csv435 BCSVView/Download
Fig 2C.csv126 BCSVView/Download
Fig 2D.csv155 BCSVView/Download
Fig 2E.csv445 BCSVView/Download
Fig 3A.csv952 BCSVView/Download
Fig 3B.csv634 BCSVView/Download
Fig 3C.csv536 BCSVView/Download
Fig 3D.csv443 BCSVView/Download
Fig 3E western blot images-repeat experiment.jpg534.73 kBJPEGThumbnail
Fig 3E western blot images.jpg607.32 kBJPEGThumbnail
Fig 3E.csv335 BCSVView/Download
Fig 4A.csv81 BCSVView/Download
Fig 4B and C-orginal images.jpg1.82 MBJPEGThumbnail
Fig 4D.csv548 BCSVView/Download
Fig 4E.csv392 BCSVView/Download
Fig 4F.csv233 BCSVView/Download
Fig 5C.csv540 BCSVView/Download
Fig 5D.csv471 BCSVView/Download
Fig 5E.csv482 BCSVView/Download
Fig 5F.csv310 BCSVView/Download
Fig 6A.csv366 BCSVView/Download
Fig 6B.csv370 BCSVView/Download
Fig 6D.csv521 BCSVView/Download
Fig 6E.csv332 BCSVView/Download
Supplementary Fig 1D.csv194 BCSVView/Download
Supplementary Fig 2-HBV DNA.csv156 BCSVView/Download
Supplementary Fig 2-HBV DNA.jpg58.86 kBJPEGThumbnail
Supplementary Fig 2-HDV RNA.csv157 BCSVView/Download
Supplementary Fig 2-HDV RNA.jpg65.17 kBJPEGThumbnail
Supplementary Fig 4A.csv154 BCSVView/Download
Supplementary Fig 4B.csv943 BCSVView/Download
Supplementary Fig 4C.csv400 BCSVView/Download

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