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Title: | Data For “Development of a dual channel detection system for pan-genotypic simultaneous quantification of hepatitis B and delta viruses" |
Contributors: | Maya, Stephanie Carver, Sebastian O’Connell, Aoife Zen, Anna Gertje, Hans Seneca, Kathleen Nahass, Ronald Crossland, Nicholas Ploss, Alexander Liu, Yongzhen |
Keywords: | hepatitis delta virus HDV hepatitis B virus HBV viral hepatitis viral detection humanized mice |
Issue Date: | 2024 |
Publisher: | Princeton University |
Abstract: | Hepatitis B virus (HBV) infection remains a major public health problem and, in associated co-infection with hepatitis delta virus (HDV), causes the most severe viral hepatitis and accelerated liver disease progression. As a defective satellite RNA virus, HDV can only propagate in the presence of HBV infection, which makes HBV DNA and HDV RNA the standard biomarkers for monitoring the virological response upon antiviral therapy, in co-infected patients. Although assays have been described to quantify these viral nucleic acids in circulation independently, a method for monitoring both viruses simultaneously is not available, thus hampering characterization of their complex dynamic interactions. Here, we describe the development of a dual fluorescence channel detection system for pan-genotypic, simultaneous quantification of HBV DNA and HDV RNA through a one-step quantitative PCR. The sensitivity for both HBV and HDV is about 10 copies per microliter without significant interference between these two detection targets. This assay provides reliable detection for HBV and HDV basic research in vitro and in human liver chimeric mice. Preclinical validation of this system on serum samples from patient on or off antiviral therapy also illustrates a promising application that is rapid and cost-effective in monitoring HBV and HDV viral loads simultaneously. |
URI: | http://arks.princeton.edu/ark:/88435/dsp01js956k14q https://doi.org/10.34770/s4zx-ab39 |
Referenced By: | https://doi.org/10.1080/22221751.2024.2350167 |
Appears in Collections: | Research DataSets |
Files in This Item:
File | Description | Size | Format | |
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20240429-DATASET_Development of a dual channel detection system for pan-genotypic simultaneous quantification of hepatitis B and delta viruses copy.txt | README file | 40.08 kB | Text | View/Download |
Fig 1B.csv | 352 B | CSV | View/Download | |
Fig 1C.csv | 362 B | CSV | View/Download | |
Fig 1D.csv | 464 B | CSV | View/Download | |
Fig 1F.csv | 506 B | CSV | View/Download | |
Fig 1G.csv | 823 B | CSV | View/Download | |
Fig 1H.csv | 732 B | CSV | View/Download | |
Fig 1I.csv | 435 B | CSV | View/Download | |
Fig 2C.csv | 126 B | CSV | View/Download | |
Fig 2D.csv | 155 B | CSV | View/Download | |
Fig 2E.csv | 445 B | CSV | View/Download | |
Fig 3A.csv | 952 B | CSV | View/Download | |
Fig 3B.csv | 634 B | CSV | View/Download | |
Fig 3C.csv | 536 B | CSV | View/Download | |
Fig 3D.csv | 443 B | CSV | View/Download | |
Fig 3E western blot images-repeat experiment.jpg | 534.73 kB | JPEG | View/Download | |
Fig 3E western blot images.jpg | 607.32 kB | JPEG | View/Download | |
Fig 3E.csv | 335 B | CSV | View/Download | |
Fig 4A.csv | 81 B | CSV | View/Download | |
Fig 4B and C-orginal images.jpg | 1.82 MB | JPEG | View/Download | |
Fig 4D.csv | 548 B | CSV | View/Download | |
Fig 4E.csv | 392 B | CSV | View/Download | |
Fig 4F.csv | 233 B | CSV | View/Download | |
Fig 5C.csv | 540 B | CSV | View/Download | |
Fig 5D.csv | 471 B | CSV | View/Download | |
Fig 5E.csv | 482 B | CSV | View/Download | |
Fig 5F.csv | 310 B | CSV | View/Download | |
Fig 6A.csv | 366 B | CSV | View/Download | |
Fig 6B.csv | 370 B | CSV | View/Download | |
Fig 6D.csv | 521 B | CSV | View/Download | |
Fig 6E.csv | 332 B | CSV | View/Download | |
Supplementary Fig 1D.csv | 194 B | CSV | View/Download | |
Supplementary Fig 2-HBV DNA.csv | 156 B | CSV | View/Download | |
Supplementary Fig 2-HBV DNA.jpg | 58.86 kB | JPEG | View/Download | |
Supplementary Fig 2-HDV RNA.csv | 157 B | CSV | View/Download | |
Supplementary Fig 2-HDV RNA.jpg | 65.17 kB | JPEG | View/Download | |
Supplementary Fig 4A.csv | 154 B | CSV | View/Download | |
Supplementary Fig 4B.csv | 943 B | CSV | View/Download | |
Supplementary Fig 4C.csv | 400 B | CSV | View/Download |
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