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http://arks.princeton.edu/ark:/88435/dsp01f4752k933
Title: | Analysis of Drosophila Virilis Chromatin Structure and its Formation During Early Embryogenesis |
Authors: | Curlin, Marcus |
Advisors: | Levine, Michael S |
Department: | Molecular Biology |
Class Year: | 2022 |
Abstract: | The 3-dimensional organization of the Drosophila virilis genome and the mechanism of its development are poorly understood. A series of technological advancements over the past decade have revolutionized the ability to study chromatin structure in the cell. In this thesis, I analyze D. virilis Micro-C and ATAC-seq data during embryogenesis to better understand the patterns of chromatin architecture and the genetic elements involved in its formation. By comparing ATAC-seq chromatin accessibility data between nc14 and later mixed-stage D. virilis embryos, I identified a number of potentially regulated genes during embryogenesis. I propose that these putative cis-regulatory regions can be useful targets for investigation on changes in chromatin structure during early embryogenesis. Additionally, I identified a pattern of heightened chromatin accessibility at topologically associating domain (TAD) borders, especially during nc14, implicating the possibility of genetic elements involved in TAD formation. Separately, I helped optimize the RD-SPRITE protocol for Drosophila virilis embryos, setting up future research to understand the role of DNA-RNA interactions in establishing 3D genomic structure during early embryogenesis. |
URI: | http://arks.princeton.edu/ark:/88435/dsp01f4752k933 |
Type of Material: | Princeton University Senior Theses |
Language: | en |
Appears in Collections: | Molecular Biology, 1954-2023 |
Files in This Item:
File | Description | Size | Format | |
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CURLIN-MARCUS-THESIS.pdf | 929.28 kB | Adobe PDF | Request a copy |
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