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Title: Investigating the sorting and intracellular transport of planar cell polarity proteins Frizzled6 and Vangl2 to and from the cell junction
Authors: Rodriguez, Daisy
Advisors: Devenport, Danelle
Department: Molecular Biology
Certificate Program: Global Health and Health Policy Program
Class Year: 2022
Abstract: Planar cell polarity (PCP) is an essential feature that describes the coordinated orientation of cell polarity across a tissue and has been implicated in mechanisms across different cell types and cell tissues. PCP is established through the asymmetric distribution of PCP components within cells, and which share interaction with PCP components on adjacent cell, creating a system for cell-to-cell communication of polarity across tissue. Disruptions in the genes which transcribe the PCP components lead to loss of uniform PCP in tissue as well as effects in development of organs, the skeletal system, and during embryogenesis. Because of its importance in development of a variety of different tissues and its implications in human health and disease, it is important to understand the mechanism for the PCP pathway, specifically how the asymmetrical localization of PCP core proteins is established. Previous studies have focused on the intracellular trafficking of core PCP proteins and found that the Frizzled6 (Fzd6) and Vangl2 proteins exit the Golgi in separate compartments guided by sorting motifs that are part of different mechanisms. Though the sorting motifs were found to be necessary for this differential compartmentalization, the question of whether they are sufficient for this was not been answered. The investigation of this differential exit of the Golgi in a polarized system like the mammalian skin also offers the ability to ask questions about its implications in asymmetric localization The mode of trajectory to and from the cell junctions is also important to investigate in an attempt to better characterize trafficking of Fzd6 and Vangl2 in the mammalian skin. The goals was to use a protein-sequestering assay to halt protein trafficking and be able to visually see how vesicle colocalize.
Type of Material: Princeton University Senior Theses
Language: en
Appears in Collections:Molecular Biology, 1954-2022
Global Health and Health Policy Program, 2017-2022

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