Skip navigation
Please use this identifier to cite or link to this item: http://arks.princeton.edu/ark:/88435/dsp018p58pg87z
Title: USE OF DESIGNER CHROMATIN SUBSTRATES FOR MECHANISTIC STUDIES OF THE HISTONE METHYLTRANSFERASE PRC2
Authors: Ge, Eva Jingjing
Advisors: Muir, Tom W
Contributors: Chemistry Department
Keywords: chromatin
epigenetics
PRC2
Subjects: Cellular biology
Biochemistry
Issue Date: 2019
Publisher: Princeton, NJ : Princeton University
Abstract: Polycomb Repressive Complex 2 (PRC2) catalyzes mono-, di-, and trimethylation of lysine 27 on histone H3 (H3K27me1-3) to control expression of genes important for differentiation and maintenance of cell identity. PRC2 activity is regulated by a number of different inputs, including allosteric activation by its product, H3K27me3. This positive feedback loop is thought to be important for the establishment of large domains of condensed heterochromatin. In addition to other chromatin modifications, ancillary subunits of PRC2, foremost JARID2, affect the rate of H3K27 methylation. Many gaps remain in our understanding of how PRC2 integrates these various signals to determine where and when to deposit H3K27 methyl marks. In this study, we utilize designer chromatin substrates to demonstrate that propagation of H3K27 methylation by the PRC2 core complex has geometrically defined preferences that are overridden by the presence of JARID2. Our studies also show that phosphorylation of JARID2 can regulate its ability to stimulate PRC2 activity. Collectively, these biochemical insights further our understanding of the mechanisms that govern PRC2 activity, and highlight a role for JARID2 in de novo deposition of H3K27me3-containing repressive domains.
URI: http://arks.princeton.edu/ark:/88435/dsp018p58pg87z
Alternate format: The Mudd Manuscript Library retains one bound copy of each dissertation. Search for these copies in the library's main catalog: catalog.princeton.edu
Type of Material: Academic dissertations (Ph.D.)
Language: en
Appears in Collections:Chemistry

Files in This Item:
This content is embargoed until 2021-10-04. For more information contact the Mudd Manuscript Library.


Items in Dataspace are protected by copyright, with all rights reserved, unless otherwise indicated.