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Title: CrvA and CrvB form a curvature-inducing module sufficient to induce cell shape complexity in Gram-negative bacteria
Contributors: Martin, Nicholas R
Blackman, Edith
Bratton, Benjamin P
Chase, Katelyn J
Bartlett, Thomas M
Gitai, Zemer
Issue Date: 2021
Publisher: Princeton University
Abstract: Bacterial species have diverse cell shapes that enable motility, colonization, and virulence. The cell wall defines bacterial shape and is primarily built by two cytoskeleton-guided synthesis machines, the elongasome and the divisome. However, the mechanisms producing complex shapes, like the curved-rod shape of Vibrio cholerae, are incompletely defined. Previous studies have reported that species-specific regulation of cytoskeleton-guided machines enables formation of complex bacterial shapes such as cell curvature and cellular appendages. In contrast, we report that CrvA and CrvB are sufficient to induce complex cell shape autonomously of the cytoskeleton in V. cholerae. The autonomy of the CrvAB module also enables it to induce curvature in the Gram-negative species Escherichia coli, Pseudomonas aeruginosa, Caulobacter crescentus, and Agrobacterium tumefaciens. Using inducible gene expression, quantitative microscopy, and biochemistry we show that CrvA and CrvB circumvent the need for patterning via cytoskeletal elements by regulating each other to form an asymmetrically-localized, periplasmic structure that directly binds to the cell wall. The assembly and disassembly of this periplasmic structure enables dynamic changes in cell shape. Bioinformatics indicate that CrvA and CrvB may have diverged from a single ancestral hybrid protein. Using fusion experiments in V. cholerae, we find that a synthetic CrvA/B hybrid protein is sufficient to induce curvature on its own, but that expression of two distinct proteins, CrvA and CrvB, promotes more rapid curvature induction. We conclude that morphological complexity can arise independently of cell shape specification by the core cytoskeleton-guided synthesis machines.
Description: The original raw data files for this project are primarily microscopy data. The files are organized into a directory structure that reflects the experiments that go into each figure and subfigure panel. Included in the README.txt are (I) a description of the file types and (II) a comma separated table documenting the folder structure and how many of each file type is in each folder.
Appears in Collections:Research Data Sets

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README.txt45.99 kBTextView/Download
Figure_1_S1_S2_Archive_A.tgz597.92 MBArchiveView/Download
Figure_1_S1_S2_Archive_B.tgz2.11 GBArchiveView/Download
Figure_1_S1_S2_Archive_C.tgz2.68 GBArchiveView/Download
Figure_2_S3A.tgz1.89 GBArchiveView/Download
Figure_3.tgz48.98 MBArchiveView/Download
Figure_4_S6D_S6E_Archive_A.tgz2.04 GBArchiveView/Download
Figure_4_S6D_S6E_Archive_B.tgz2.12 GBArchiveView/Download
Figure_5_S7A_Archive_A.tgz803.55 MBArchiveView/Download
Figure_5_S7A_Archive_B.tgz2.76 GBArchiveView/Download
Figure_5_S7A_Archive_C.tgz2.82 GBArchiveView/Download
Figure_5_S7A_Archive_D.tgz2.85 GBArchiveView/Download
Figure_6_Archive_A.tgz945.96 MBArchiveView/Download
Figure_6_Archive_B.tgz1.94 GBArchiveView/Download
Figure_S3.tgz602.65 MBArchiveView/Download
Figure_S4.tgz2.3 GBArchiveView/Download
Figure_S5.tgz230.8 MBArchiveView/Download
Figure_S7.tgz391.55 MBArchiveView/Download
Figure_S8.tgz1.23 GBArchiveView/Download
Supplemental_movies.tgz536.33 MBArchiveView/Download

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