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Please use this identifier to cite or link to this item: http://arks.princeton.edu/ark:/88435/dsp018c97kt64b
Title: Data and code for “Structured foraging of soil predators unveils functional responses to bacterial defenses”
Contributors: Vercelli, Gabriel
Tarnita, Corina
Gregor, Thomas
Rossine, Fernando
Keywords: quantitative microscopy
predator-prey
single-cell measurements
modeling
Issue Date: 11-Nov-2022
Publisher: Princeton University
Abstract: Microscopy images are part of a paper entitled "Structured foraging of soil predators unveils functional responses to bacterial defenses" by Fernando Rossine, Gabriel Vercelli, Corina Tarnita, and Thomas Gregor. For detailed acquisition methods see the paper. Experiments were performed between 2019 and 2020 at Princeton University. Two types of images are provided, macroscopic and microscopic widefiled Images. Macroscopic images all show Petri dishes covered in fluorescent bacteria being consumed by amoebae. Images are shown for D. discoideum, P. violaceum, and A. castellanii. Images depicting drug treatments (Nystatin and Fluorouracil) were obtained using D. discoideum. Images used for the creation of a profile were all taken within 30 minutes of each other. Within each directory numbered images are independent replicates. The raw video directory contains time series for dishes under drug treatments. Each numbered folder is a sequence of photos (taken 30 minutes apart of each other) of a single dish. Microscopic images all show amoebae consuming bacteria on a petri dish. The 45 minute videos show either edge cells (located at the edge of amoebae colonies), or inner cells (located 2.5 millimeters towards the center of the colony, from the edge). Videos are confocal stacks, with bacteria showing in green and amoebae appearing as black holes within the bacterial lawn. As was for the macroscopic images, images are shown for D. discoideum, P. violaceum, and A. castellanii. Images depicting drug treatments (Nystatin and Fluorouracil) were obtained using D. discoideum.
Description: This dataset is too large to download directly from this item page. You can access and download the data via Globus at this link: https://app.globus.org/file-manager?destination_id=dc43f461-0ca7-4203-848c-33a9fc00a464&destination_path=%2F5cey-ce46%2F
URI: http://arks.princeton.edu/ark:/88435/dsp018c97kt64b
https://doi.org/10.34770/5cey-ce46
https://app.globus.org/file-manager?destination_id=dc43f461-0ca7-4203-848c-33a9fc00a464&destination_path=%2F5cey-ce46%2F
Referenced By: Rossine FW, Vercelli G, Tarnita C, Gregor T. Structured foraging of soil predators unveils functional responses to bacterial defenses. Proceedings of the National Academy of Science (in press).
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