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Title: Cell Adhesion for Improved Prosthetics: Determining Spatial Parameters to Allow Cell Spreading to Reach New Heights
Authors: Merewether, Gene B.
Advisors: Schwartz, Jeffrey
Department: Chemistry
Class Year: 2013
Abstract: Currently, there exists a mismatch between titanium bone replacement implants and native bone. Polyetheretherketone (PEEK) is favored as a replacement, but cells do not adhere well to untreated PEEK. This work attempts to combine 3D printing, materials properties, surface treatments, and control of structure geometries to test: 1. Is 3D printing a viable route to medical devices that promote cell in- and on-growth? 2. What dimensions allow cells to climb and perfuse on and in an entire engineered structure? To answer these questions, a porous PEEK scaffold with 800μm channels was constructed by selective laser sintering (SLS) and inspected visually and by scanning electron microscopy (SEM) for proper formation. The results indicate that these dimensions are near the minimum viable dimensions for channels in SLS PEEK, while still building successfully. A thin zirconium oxide layer was formed on the PEEK surface by chemical vapor deposition (CVD), followed by formation of a self-assembled monolayer (SAM) of diphosphonic acid. X-ray photoelectron spectroscopy (XPS) analysis of surfaces of sections of the device indicated treatment success in the device interior. Computer simulations of the discrepancy between the design of 3D parts and the finished SLS products were carried out. To assay the tolerance of cells to microstructure of devices, PEEK cell migration devices were constructed using standard machining techniques. Cell adhesion and migration were assayed on plain PEEK, zirconated PEEK, and phosphonated PEEK. Cells adhered the farthest from the center of the devices on the phosphonated surface, second farthest on the zirconated surface, and least far on plain PEEK. This work serves to characterize the parameters of manufacturing methods viable for producing bone replacement prosthetics. The influence of surface characteristics and celladhesive surface treatments on cell adhesion and migration are examined, hopefully leading to improved implant fixation and customizable shape.
Extent: 68 pages
Access Restrictions: Walk-in Access. This thesis can only be viewed on computer terminals at the Mudd Manuscript Library.
Type of Material: Princeton University Senior Theses
Language: en_US
Appears in Collections:Chemistry, 1926-2017

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