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Title: Establishment and maintenance of rod shape by the bacterial actin homologue MreB
Authors: Ouzounov, Nikolay
Advisors: Shaevitz, Joshua W
Contributors: Molecular Biology Department
Keywords: Bacteria
Cell Shape
Subjects: Molecular biology
Issue Date: 2015
Publisher: Princeton, NJ : Princeton University
Abstract: Bacteria have evolved a wide range of morphologies. For the majority of bacteria, the peptidoglycan (PG) cell wall denes their cell shape. Although many of the proteins responsible for synthesizing the PG cell wall are known, the mechanisms of how they are spatiotemporally regulated remains unknown. For the most part, these enzymes are hypothesized to exist in a cell wall synthesis complex. The most likely candidate for organizing this cell wall synthesis complex is the bacterial actin homologue MreB. The structure of MreB polymers and how they organize cell wall synthesis have only recently begun to be evaluated. In this work, I use a combination of novel functional uorescent protein fusion to MreB at mreB's native locus along with novel 2D and 3D cell shape analysis of uorescence images. Using these techniques I rst show that MreB has preferential membrane curvature localization and discuss how this is related to rod shape maintenance. Next I explore how MreB is necessary for rod shape establishment through the examination of growth and reversion of cell wall decient E. coli. Finally, I explore the role of MreB polymers in how bacterial cells determine their radius. This work shows the multiple functions MreB polymers play in the organization of cell wall synthesis and brings new insights into how bacteria establish and maintain their rod shape.
Alternate format: The Mudd Manuscript Library retains one bound copy of each dissertation. Search for these copies in the library's main catalog
Type of Material: Academic dissertations (Ph.D.)
Language: en
Appears in Collections:Molecular Biology

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