Skip navigation
Please use this identifier to cite or link to this item: http://arks.princeton.edu/ark:/88435/dsp010k225d376
Title: Ribosome Profiling and Translational Control in Drosophila Embryo and Ovary Tissues
Authors: Shi, Kevin
Advisors: Gavis, Elizabeth
Department: Molecular Biology
Class Year: 2015
Abstract: The newly developed method of ribosome profiling allows for the direct examination of translational regulatory events. We have extended a ribosome profiling experiment done in Drosophila embryos into ovary samples. Though some of our analyses were confounded by sample noise, likely owing to relatively low sequencing depth, we have identified novel potential translational regulation events through the detection of aberrant ribosome coverage in numerous genes through both low resolution averages of ribosome profile and higher resolution nucleotide-level analyses. Finally, following previous experiments that have suggested a readthrough event in par-1, we have determined that the 3’ UTR of par-1 contains a nuclear localization signal, such that a readthrough event in par-1 would lead to its sequestration in the nucleus.
Extent: 77 pages
URI: http://arks.princeton.edu/ark:/88435/dsp010k225d376
Type of Material: Princeton University Senior Theses
Language: en_US
Appears in Collections:Molecular Biology, 1954-2016

Files in This Item:
File SizeFormat 
PUTheses2015-Shi_Kevin.pdf1.37 MBAdobe PDF    Request a copy


Items in Dataspace are protected by copyright, with all rights reserved, unless otherwise indicated.