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Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yang, Haw | - |
dc.contributor.author | Studenikina, Alev | - |
dc.date.accessioned | 2023-07-25T12:58:58Z | - |
dc.date.available | 2023-07-25T12:58:58Z | - |
dc.date.created | 2023-04-17 | - |
dc.date.issued | 2023-07-25 | - |
dc.identifier.uri | http://arks.princeton.edu/ark:/88435/dsp015x21tj71m | - |
dc.description.abstract | Modern-day spectroscopy is capable of providing high-resolution, non-diffraction-limited images of the cellular interior. Using multiple fluorophores, biological processes such as vesicular transport, organelle remodelling and even protein-protein interactions can be visualized. However, the single-molecule perspective, crucial for understanding the spatially and temporally heterogeneous intracellular environment, is missing. While single-molecule and single-particle tracking, including inside living cells, has become routine, a lot of chemical information is lost because of the short observation time of the least intrusive fluorescent labels: organic fluorophores. These are prone to photobleaching: irreversible reactions of the excited state that completely quench the fluorescence of a molecule. Presented here are the initial stages of the experimental investigation of a proposed platform for overcoming this limitation by replacing a spent probe in situ. This proof-of-concept nanoplatform involves a dye/quencher-labelled DNA hairpin that can be added to and removed from target in a controlled fashion. The DNA hairpin fluctuations between the ‘open’ and the ‘closed’ state when bound to target can be observed by single-molecule F¨orster-type Resonance Energy Transfer (smFRET). Demonstrating that this design works experimentally with a trackable nanoparticle as the target will open doors to carrying out extended single-molecule fluorescence + tracking experiments in vitro and potentially in vivo, allowing rare biological events to be observed. | en_US |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | en_US |
dc.title | Developing a DNA-based nanoplatform for extended single-molecule fluorescence measurements with potential applications for in vivo studies | en_US |
dc.type | Princeton University Senior Theses | |
pu.date.classyear | 2023 | en_US |
pu.department | Chemistry | en_US |
pu.pdf.coverpage | SeniorThesisCoverPage | |
pu.contributor.authorid | 920226328 | |
pu.mudd.walkin | No | en_US |
Appears in Collections: | Chemistry, 1926-2023 |
Files in This Item:
File | Description | Size | Format | |
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STUDENIKINA-ALEV-THESIS.pdf | 1.64 MB | Adobe PDF | Request a copy |
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